cytiva AKTA pure Mode d'emploi

To eliminate air bubbles, prime the inlets and purge the pump heads. Fill all inlet tubings with the appropriate buffer, ensuring all inlet tubings to be used are immersed in the correct buffer. Purge the system pumps to remove air from the pump heads by connecting a syringe to the purge valve of each pump head and slowly drawing liquid until it reaches the pump, then close the valve and disconnect the syringe. Ensure no column is online during these operations. Regularly check the flow restrictor and clean the check valves if necessary, as contaminant build-up can also affect the correct functioning of the system.

To clean the ÄKTA pure system after protein purification, use the System CIP function to rinse the instrument with cleaning solutions (sodium hydroxide, buffer, distilled water). Also perform a Column CIP with appropriate solutions. For general maintenance, empty the fraction collector and waste container, clean spills and the manual injection port. If necessary, manually clean the pH measuring electrode. If dangerous chemicals have been used, it is imperative to rinse the entire flow path with distilled water before any maintenance.

Check the status lights on the control panel: a flashing white light indicates a communication issue, a steady orange light means the cycle is paused, and a flashing red light indicates an alarm or error. If air is trapped in the pump heads, perform priming and purging. Additionally, verify the system status in the UNICORN software and ensure the power cord is properly connected.

1. Check status lights on the control panel: flashing white indicates a communication error, steady orange indicates the system is paused, and flashing red indicates an alarm. 2. Verify the system state in the UNICORN software to see if it is in Pause or Hold mode. 3. If air is present in the pump heads, perform priming and purging using a syringe. 4. Ensure the power switch is in the 'I' position and the cord is connected.

Check the status lights (white for communication, orange for pause, red for alarm), verify the system status in the UNICORN software, and perform priming/purging of the pump heads to remove air.

Check the status lights: a slow flashing white light indicates a communication error, a steady orange light means the cycle is paused, and a flashing red light indicates a system alarm. Also, verify the status in the UNICORN software System Control module and check for air in the pump heads, which may require priming.